广东工业大学学报 ›› 2022, Vol. 39 ›› Issue (02): 130-136.doi: 10.12052/gdutxb.200170

• 综合研究 • 上一篇    

IrO2@MnO2纳米酶的制备及用于抗坏血酸的检测

杨俊松, 钟颖颖, 余倩   

  1. 广东工业大学 轻工化工学院, 广东 广州 510006
  • 收稿日期:2020-12-23 出版日期:2022-03-10 发布日期:2022-04-02
  • 通信作者: 余倩(1964-)，女，教授，主要研究方向为催化、材料、精细化工和环境检测等，E-mail：821833656@qq.com
  • 作者简介:杨俊松(1996-)，男，硕士研究生，主要研究方向为无机纳米材料合成
  • 基金资助:
    国家自然科学基金资助面上项目(21576054)

Preparation of IrO2@MnO2 Nanozyme and Its Application in the Detection of Ascorbic Acid

Yang Jun-song, Zhong Ying-ying, Yu Qian   

  1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China
  • Received:2020-12-23 Online:2022-03-10 Published:2022-04-02

HTML

PDF (PC)

2377

摘要： 通过原位整合法制备了粒径约为136 nm，形态均一的方块状IrO2@MnO2纳米复合物。经实验证明该复合物具有协同提高的氧化酶模拟活性，其Km值为6.50 × 10-3 mmol/L，远优于IrO2 纳米颗粒(IrO2 Nanoparticles，IrO2 NPs)(Km = 9.73 × 10-2 mmol/L)和MnO2纳米颗粒(MnO2 Nano-particles, MnO2 NPs)(Km = 2.41 × 10-1 mmol/L)。此外，通过实验获得了用于抗坏血酸(AscorbicAcids，AA)检测的IrO2@MnO2纳米复合物的最佳传感条件，包括反应体系为pH为4、反应温度为37 ℃、反应时间为5 min、IrO2@MnO2复合纳米酶质量浓度为71.43 μg/mL。最后，IrO2@MnO2纳米复合物被用于检测AA，检测范围为0~312.5 μmol/L，定量限为14.2 μmol/L，检测限为1.42 μmol/L。本文所制备IrO2@MnO2纳米酶在食品监制、生物医药、临床诊断等领域具有较高的潜在应用价值。

关键词: 氧化酶样纳米酶, 抗坏血酸检测, 二氧化铱, 二氧化锰

Abstract: A uniform square shaped IrO2@MnO2 nanocomposite with a particle size of about 136 nm was prepared by the in-situ integration method. Results show that the nanocomposite has synergistically improved oxidase mimic activity, and its Kmvalue is 6.50 × 10-3 mmol/L which is better than that of IrO2 NPs (Km = 9.73 × 10-2 mmol/L) and MnO2 NPs (Km = 2.41 × 10-1 mmol/L), respectively. In addition, the optimal sensing conditions of IrO2@MnO2 nanocomposite for AA detection are obtained through experiments, which include the reaction system at pH 4, the reaction temperature at 37 ℃, the reaction time at 5 min, and the concentration of IrO2@MnO2 nanocomposite at 71.43 μg/mL. Finally, the IrO2@MnO2 nanocomposite is used for sensitive detection of AA, the detection range is 0~312.5 μmol/L, thequantification limit is 14.2 μmol/L and the detection limit is 1.42 μmol/L.

Key words: oxidase-like nanozyme, ascorbic acid detection, iridium dioxide, manganese dioxide

中图分类号: 

  • O657.3
[1] ZHANG H J, CHEN J, YANG Y L, et al. Discriminative detection of glutathione in cell lysates based on oxidase-like activity of magnetic nanoporous graphene [J]. Analytical Chemistry, 2019, 91(8): 5004-5010.
[2] JIN L H, SUN Y N, SHI L L, et al. PdPt bimetallic nanowires with efficient oxidase mimic activity for the colorimetric detection of acid phosphatase in acidic media [J]. Journal of Materials Chemistry B, 2019, 7(29): 4561-4567.
[3] HAYAT A, CUNNINGHAM J, BULBUL G, et al. Evaluation of the oxidase like activity of nanoceria and its application in colorimetric assays [J]. Analytica Chimica Acta, 2015, 885: 140-147.
[4] HANSAEM J, JAEYOUNG L. Iridium oxide fabrication and application: a review [J]. Journal of Energy Chemistry, 2020, 46(7): 164-184.
[5] XU D, DIAO P, JIN T, et al. Iridium oxide nanoparticles and iridium/iridium oxide nanocomposites: photochemical fabrication and application in catalytic reduction of 4-nitrophenol [J]. ACS Applied Materials & Interfaces, 2015, 7(30): 16738-16749.
[6] QUESADA-GONZÁLEZ D, BAIOCCO A, MARTOS A A, et al. Iridium oxide (IV) nanoparticle-based electrocatalytic detection of PBDE [J]. Biosensors & Bioelectronics, 2019, 127: 150-154.
[7] SHIVA KUMAR S, HIMABINDU V. Hydrogen production by PEM water electrolysis: a review [J]. Material Science for Energy Technology, 2019, 2(3): 442-454.
[8] CARMO M, FRITZ D L, MERGEL J, et al. A comprehensive review on PEM water electrolysis [J]. International Journal of Hydrogen Energy, 2013, 38(12): 4901-4934.
[9] ANTOLINI E. Iridium as catalyst and cocatalyst for oxygen evolution/reduction in acidic polymer electrolyte membrane electrolyzers and fuel cells [J]. ACS Catalysis, 2014, 4(5): 1426-1440.
[10] QUESADA-GONZÁLEZ D, SENA-TORRALBA A, WICAKSONO W P, et al. Iridium oxide (IV) nanoparticle-based lateral flow immunoassay [J]. Biosensors and Bioelectronics, 2019, 132: 132-135.
[11] WANG Q B, ZHANG C J, YU H, et al. The sensitive "Turn-on" fluorescence platform of ascorbic acid based on conjugated polymer nanoparticles [J]. Analytica Chimica Acta, 2020, 1097: 153-160.
[12] ABELLÁN-LLOBREGAT A, VIDAL L, RODRÍGUEZ-AMARO R, et al. Evaluation of herringbone carbon nanotubes-modified electrodes for the simultaneous determination of ascorbic acid and uric acid [J]. Electrochimica Acta, 2018, 285: 284-291.
[13] XIE Z, SUN X, JIAO J, et al. Ionic liquid-functionalized carbon quantum dots as fluorescent probes for sensitive and selective detection of iron ion and ascorbic acid [J]. Colloid and Surface A: Physicochemical and Engineering Aspects, 2017, 529: 38-44.
[14] MOTSHAKERI M, TRAVAS-SEJDIC J, PHILLIPS A R J, et al. Rapid electroanalysis of uric acid and ascorbic acid using a poly (3, 4-ethylenedioxythiophene)-modified sensor with application to milk [J]. Electrochimica Acta, 2018, 265: 184-193.
[15] ABELLAN-LLOBREGAT A, GONZALEZ-GAITAN C, VIDAL L, et al. Portable electrochemical sensor based on 4-aminobenzoic acid-functionalized herringbone carbon nanotubes for the determination of ascorbic acid and uric acid in human fluids [J]. Biosensors & Bioelectronics, 2018, 109: 123-131.
[16] LI Y, LIN H C, PENG H, et al. A glassy carbon electrode modified with MoS2 nanosheets and poly (3, 4-ethylenedioxythiophene) for simultaneous electrochemical detection of ascorbic acid, dopamine and uric acid [J]. Microchimica Acta, 2016, 183: 2517-2523.
[17] ZHANG G, REN L, YAN Z, et al. Rational design and controllable preparation of holey MnO2 nanosheets [J]. Chemical Communications, 2017, 53(20): 2950-2953.
[18] KURBANOGLU S, RIVAS L, OZKAN S A, et al. Electrochemically reduced graphene and iridium oxide nanoparticles for inhibition-based angiotensin-converting enzyme inhibitor detection [J]. Biosensors and Bioelectronics, 2017, 88: 122-129.
[19] LIU X, WANG X, HAN Q, et al. Facile synthesis of IrO2/rGO nanocomposites with high peroxidase-like activity for sensitive colorimetric detection of low weight biothiols [J]. Talanta, 2019, 203: 227-234.
[20] YAN X, SONG Y, WU X, et al. Oxidase-mimicking activity of ultrathin MnO2 nanosheets in colorimetric assay of acetylcholinesterase activity [J]. Nanoscale, 2017, 9: 2317-2323.
[1] 周祖卫, 余倩, 郝志峰, 张佐宏, 罗银好, 余林, 戴立松. 无纺布负载锰氧化物的制备及其催化分解甲醛性能研究[J]. 广东工业大学学报, 2018, 35(02): 6-10.
[2] 符志伟，程高，林婷，孙明，余林. 二氧化锰制备及催化燃烧甲苯性能研究[J]. 广东工业大学学报, 2016, 33(02): 85-90.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2017 《广东工业大学学报》编辑部
地址：广州市东风东路729号广东工业大学 邮编：510090
电话：020-37626653,020-37626139,020-37626396
邮箱：xbzrb@gdut.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发