广东工业大学学报 ›› 2022, Vol. 39 ›› Issue (02): 130-136.doi: 10.12052/gdutxb.200170

• 综合研究 • 上一篇    

IrO2@MnO2纳米酶的制备及用于抗坏血酸的检测

杨俊松, 钟颖颖, 余倩   

  1. 广东工业大学 轻工化工学院, 广东 广州 510006
  • 收稿日期:2020-12-23 出版日期:2022-03-10 发布日期:2022-04-02
  • 通信作者: 余倩(1964-)，女，教授，主要研究方向为催化、材料、精细化工和环境检测等，E-mail：821833656@qq.com
  • 作者简介:杨俊松(1996-)，男，硕士研究生，主要研究方向为无机纳米材料合成
  • 基金资助:
    国家自然科学基金资助面上项目(21576054)

Preparation of IrO2@MnO2 Nanozyme and Its Application in the Detection of Ascorbic Acid

Yang Jun-song, Zhong Ying-ying, Yu Qian   

  1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China
  • Received:2020-12-23 Online:2022-03-10 Published:2022-04-02

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摘要： 通过原位整合法制备了粒径约为136 nm，形态均一的方块状IrO2@MnO2纳米复合物。经实验证明该复合物具有协同提高的氧化酶模拟活性，其Km值为6.50 × 10-3 mmol/L，远优于IrO2 纳米颗粒(IrO2 Nanoparticles，IrO2 NPs)(Km = 9.73 × 10-2 mmol/L)和MnO2纳米颗粒(MnO2 Nano-particles, MnO2 NPs)(Km = 2.41 × 10-1 mmol/L)。此外，通过实验获得了用于抗坏血酸(AscorbicAcids，AA)检测的IrO2@MnO2纳米复合物的最佳传感条件，包括反应体系为pH为4、反应温度为37 ℃、反应时间为5 min、IrO2@MnO2复合纳米酶质量浓度为71.43 μg/mL。最后，IrO2@MnO2纳米复合物被用于检测AA，检测范围为0~312.5 μmol/L，定量限为14.2 μmol/L，检测限为1.42 μmol/L。本文所制备IrO2@MnO2纳米酶在食品监制、生物医药、临床诊断等领域具有较高的潜在应用价值。

关键词: 氧化酶样纳米酶, 抗坏血酸检测, 二氧化铱, 二氧化锰

Abstract: A uniform square shaped IrO2@MnO2 nanocomposite with a particle size of about 136 nm was prepared by the in-situ integration method. Results show that the nanocomposite has synergistically improved oxidase mimic activity, and its Kmvalue is 6.50 × 10-3 mmol/L which is better than that of IrO2 NPs (Km = 9.73 × 10-2 mmol/L) and MnO2 NPs (Km = 2.41 × 10-1 mmol/L), respectively. In addition, the optimal sensing conditions of IrO2@MnO2 nanocomposite for AA detection are obtained through experiments, which include the reaction system at pH 4, the reaction temperature at 37 ℃, the reaction time at 5 min, and the concentration of IrO2@MnO2 nanocomposite at 71.43 μg/mL. Finally, the IrO2@MnO2 nanocomposite is used for sensitive detection of AA, the detection range is 0~312.5 μmol/L, thequantification limit is 14.2 μmol/L and the detection limit is 1.42 μmol/L.

Key words: oxidase-like nanozyme, ascorbic acid detection, iridium dioxide, manganese dioxide

中图分类号: 

  • O657.3
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