广东工业大学学报 ›› 2012, Vol. 29 ›› Issue (3): 81-86.doi: 10.3969/j.issn.1007-7162.2012.03.016

• 综合研究 • 上一篇    下一篇

两组不同冷冻剂组合冷冻Wistar大鼠胚胎效果的比较

 张丽1，谭竹钧1，陈炜2，高珊2，刘牧2   

  1. 1.广东工业大学 轻工化工学院，广东 广州 510006；2.中国医学科学院 医学实验动物研究所，北京 100021
  • 收稿日期:2012-01-04 出版日期:2012-09-20 发布日期:2012-09-20
  • 作者简介:张丽（1986-）,女，硕士研究生，主要研究方向为低温生物学、大鼠胚胎库.
  • 基金资助:

    卫生部行业科研专项资金资助项目(20080236)

Comparison of Cryopreservation of Wistar Rat Embryos between Two Groups of Extenders

Zhang Li1, Tan Zhujun1, Chen Wei2, Gao Shan2， Liu Mu2   

  • Received:2012-01-04 Online:2012-09-20 Published:2012-09-20

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摘要： 采用冻存管方法，用 DAP213和SDP0323两种冻存液组合分别对Wistar大鼠2-细胞期胚胎进行玻璃化冷冻，两种冻存液冷冻的胚胎平均复苏率分别为57.1%、69.1%；平均产仔率分别为34.9%、41.1%.SDP0323 冻存液冷冻的胚胎平均复苏率及平均产仔率均比DAP213冻存液冷冻的相对高一些.经统计学分析，两者之间无显著差异（P>0.05）.两组冷冻试剂组合均可作为对 Wistar大鼠2-细胞期胚胎进行冷冻保存的有效方法，可用于建立大鼠胚胎库.

关键词: 大鼠；胚胎；玻璃化冷冻；移植

Abstract: The tube method was used to study two different extenders to cryopreserve by vitrification the 2-cell Wistar Rat embryos, which were DAP213 and SDP0323. And the effects of different extenders on the average survival rates of frozenthawed embryos and on the average rates of the new borns were discussed. The average survival rates and average new born rates after being frozenthawed were 57.1%,69.1% and 34.9%,41.1% respectively. There are no significant differences between the two extenders using the Student' t-Test, but both the average survival rates and average new born rates with SDP0323 are higher than those with DAP213. The results show that the tube method with DAP213 and SDP0323 for cryopreservation of the 2-cell rat embryos has been successful, and that the two extenders are suitable for the rat 2-cell embryos-bank.

Key words: rat；embryo；vitrification cryopreservation；transplantation

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