Journal of Guangdong University of Technology ›› 2012, Vol. 29 ›› Issue (3): 81-86.doi: 10.3969/j.issn.1007-7162.2012.03.016

• Comprehensive Studies • Previous Articles     Next Articles

Comparison of Cryopreservation of Wistar Rat Embryos between Two Groups of Extenders

Zhang Li1, Tan Zhujun1, Chen Wei2, Gao Shan2， Liu Mu2   

  • Received:2012-01-04 Online:2012-09-20 Published:2012-09-20

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Abstract: The tube method was used to study two different extenders to cryopreserve by vitrification the 2-cell Wistar Rat embryos, which were DAP213 and SDP0323. And the effects of different extenders on the average survival rates of frozenthawed embryos and on the average rates of the new borns were discussed. The average survival rates and average new born rates after being frozenthawed were 57.1%,69.1% and 34.9%,41.1% respectively. There are no significant differences between the two extenders using the Student' t-Test, but both the average survival rates and average new born rates with SDP0323 are higher than those with DAP213. The results show that the tube method with DAP213 and SDP0323 for cryopreservation of the 2-cell rat embryos has been successful, and that the two extenders are suitable for the rat 2-cell embryos-bank.

Key words: rat；embryo；vitrification cryopreservation；transplantation

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