Journal of Guangdong University of Technology ›› 2021, Vol. 38 ›› Issue (02): 88-93.doi: 10.12052/gdutxb.200028

• Comprehensive Studies • Previous Articles     Next Articles

A Study of Eugenol Anti-Malassezia Activity, Cytotoxicity and Antifungal Mechanism

Liu Di1, Guo Qing-quan1, Zhao Hai-Shan2, Shu Jun-zhong3, Lian Ying-duo3, Xia Shu-min3   

  1. 1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China;
    2. School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China;
    3. Opal Cosmetics (Huizhou) Co.Ltd., Huizhou 516255, China
  • Received:2020-02-20 Online:2021-03-10 Published:2021-01-13

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Abstract: The in vitro susceptibility test was used to evaluate the activity of eugenol against Malassezia, and the interaction between eugenol and traditional antidandruff drugs was studied by the checkerboard microdilution method. The minimum inhibitory concentration (MIC) of eugenol was 312.50 μg/mL and the minimum fungicidal concentration was 625.00 μg/mL. Eugenol combined with ketoconazole showed a synergistic effect, and combined with zinc pyrithione (ZPT), climbazole, and piroctone olamine (OCT) respectively showed additive effects. Combined use can reduce the minimum effective concentration of each drug by 50%~75%. The toxicity of eugenol to keratinocytes was evaluated by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromid) assay. The results showed that the IC50 of eugenol was 320.93 mg/L, which is higher than that of traditional antidandruff agents. This indicated that eugenol has higher safety at the cellular level. The mechanism of eugenol against Malassezia was studied by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). It was found that eugenol achieves fungicidal ability by destroying cell wall and internal membrane structure of Malassezia. These results provided a theoretical basis for the application of eugenol in antidandruff products.

Key words: anti-dandruff, eugenol, Malassezia, medicine combination, cytotoxicity, antifungal mechanism

CLC Number: 

  • TQ658.3
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