Abstract:

The head, bones, and other raw waste of Tilapia are hydrolyzed by elastase, and response surface analysis method is used to optimize the enzymatic processes. The enzyme concentration, temperature, pH, solid to liquid ratio, time and other factors are discussed to analyze the effect of hydrolysis based on the degree of hydrolysis and peptide content. The results show that the optimum conditions of elastase hydrolysis: enzyme concentration 0.553 mg/g, temperature 41.94 ℃, time 3.31 h, pH natural, solid-liquid ratio 1: 5, the degree of hydrolysis and the peptide content is separately 18.09% and 0.90 mg /ml in this condition.

Key words: Tilapia waste; response surface methodology; elastase；hydrolysis; peptide