Journal of Guangdong University of Technology ›› 2018, Vol. 35 ›› Issue (06): 95-99.doi: 10.12052/gdutxb.180050

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Application of Tissue Engineering in Evaluating Anti-aging Effect of Compound Oral Liquid of Astragalus

Huang Bo-xin1, Du Zhi-yun1, Tang Ya-dong1, Dong Chang-zhi2, Zheng Ya-ting1   

  1. 1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China;
    2. ITODYS Lab, Paris Diderot, Paris 75013, France
  • Received:2018-03-19 Online:2018-11-23 Published:2018-09-12

Abstract: To investigate the anti-aging effect of compound oral liquid of astragalus on skin, SD rats were randomly divided into medicated serum group and non medicated serum group and given compound oral liquid of astragalus and saline solution at the dose of 10 ml·kg-1 once a day by intragastrical administration for 7 days, respectively. Firstly, 10% serum was added to senescence of human fibroblasts model which had been treated with 200 nM mitomycin (MMC) for 48 h. The experiment confirmed that medicated serum group has a significant role in promoting the proliferation of fibroblasts. Secondly, the expression of filaggrin in Episkin and collagen types I and Ⅲ in three-dimensional dermal aging model was evaluated after being treated with serum by immunofluorescence staining and Elisa kit, respectively. The results shows that compound astragalus oral liquid is helpful to the expression of filaggrin in epidermis to improve the function of skin barrier and water retention capacity, and collagen types I and Ⅲ to enhance mechanical properties of skin and reduce wrinkles. In conclusion, compound oral liquid of astragalus shows good effect of anti-aging on skin.

Key words: compound oral liquid of astragalus, serum, tissue engineered skin, anti-aging

CLC Number: 

  • Q811
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