广东工业大学学报 ›› 2018, Vol. 35 ›› Issue (06): 95-99.doi: 10.12052/gdutxb.180050

• • 上一篇    下一篇

组织工程皮肤在复方黄芪口服液延缓皮肤衰老研究中的应用

黄博鑫1, 杜志云1, 汤亚东1, 董长治2, 郑雅婷1   

  1. 1. 广东工业大学 轻工化工学院, 广东 广州 510006;
    2. 巴黎第七大学 ITODYS实验室, 法国 巴黎 75013
  • 收稿日期:2018-03-19 出版日期:2018-11-23 发布日期:2018-09-12
  • 通信作者: 杜志云(1969-),男,教授,博士,主要研究方向为组织工程与功能食品及功能化妆品.E-mail:zhiyundu@foxmail.com E-mail:zhiyundu@foxmail.com
  • 作者简介:黄博鑫(1993-),男,硕士研究生,主要研究方向为组织工程.
  • 基金资助:
    广东省药食同源资源综合利用工程技术中心（15ZK0213）

Application of Tissue Engineering in Evaluating Anti-aging Effect of Compound Oral Liquid of Astragalus

Huang Bo-xin1, Du Zhi-yun1, Tang Ya-dong1, Dong Chang-zhi2, Zheng Ya-ting1   

  1. 1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China;
    2. ITODYS Lab, Paris Diderot, Paris 75013, France
  • Received:2018-03-19 Online:2018-11-23 Published:2018-09-12

HTML

PDF (PC)

2571

摘要： 为探究复方黄芪口服液对皮肤衰老的延缓功效，将SD大鼠随机分为含药血清组与不含药血清组，分别采用10 mL/kg复方黄芪口服液和生理盐水灌胃SD大鼠7 d，每天1次，获取血清后将10%血清分别作用于200 nmol丝裂霉素（MMC）干预的人成纤维细胞衰老模型，验证了含药血清可有效促进细胞增殖. 再利用组织工程皮肤Episkin表皮模型和三维真皮衰老模型，分别通过免疫荧光染色与Elisa试剂盒评价血清干预后表皮模型丝聚蛋白与三维真皮中人胶原蛋白I、Ⅲ型的含量. 实验结果表明，服用复方黄芪口服液有助于表皮层丝聚蛋白与真皮层人胶原蛋白I、Ⅲ型蛋白的表达，从而提高皮肤屏障与保水能力，提升皮肤支撑力，减少皱纹生成，达到延缓皮肤衰老的功效.

关键词: 复方黄芪口服液, 血清, 组织工程皮肤, 抗衰老

Abstract: To investigate the anti-aging effect of compound oral liquid of astragalus on skin, SD rats were randomly divided into medicated serum group and non medicated serum group and given compound oral liquid of astragalus and saline solution at the dose of 10 ml·kg-1 once a day by intragastrical administration for 7 days, respectively. Firstly, 10% serum was added to senescence of human fibroblasts model which had been treated with 200 nM mitomycin (MMC) for 48 h. The experiment confirmed that medicated serum group has a significant role in promoting the proliferation of fibroblasts. Secondly, the expression of filaggrin in Episkin and collagen types I and Ⅲ in three-dimensional dermal aging model was evaluated after being treated with serum by immunofluorescence staining and Elisa kit, respectively. The results shows that compound astragalus oral liquid is helpful to the expression of filaggrin in epidermis to improve the function of skin barrier and water retention capacity, and collagen types I and Ⅲ to enhance mechanical properties of skin and reduce wrinkles. In conclusion, compound oral liquid of astragalus shows good effect of anti-aging on skin.

Key words: compound oral liquid of astragalus, serum, tissue engineered skin, anti-aging

中图分类号: 

  • Q811
[1] NEWTON V L, MCCONNELL J C, HIBBERT S A, et al. Skin aging:molecular pathology, dermal remodelling and the imaging revolution[J]. Giornale Italiano Di Dermatologia E Venereologia, 2015, 150(6):665-674
[2] RITTIÉ L, FISHER G J. Natural and sun induced aging of human skin[J]. Cold Spring Harbor Perspectives in Medicine, 2015, 5(1):a015370
[3] DOS S M, METRAL E, BOHER A, et al. In vitro 3-D model based on extending time of culture for studying chronological epidermis aging[J]. Matrix Biology Journal of the International Society for Matrix Biology, 2015, 47:85-97
[4] 钟建桥, 胡念芳, 李利. 真皮干细胞与皮肤衰老关系的研究进展[J]. 中国皮肤性病学杂志, 2010, 24(5):479-48 ZHONG J Q, HU N F, LI L. Advance in the relation between dermal stem cells and skin aging[J]. The Chinese Journal of Dermatovenereology, 2010, 24(5):479-48
[5] 宋小平, 陈志武, 方安宁, 等. 银杏叶总黄酮延缓细胞衰老的实验研究[J]. 中药材, 2009, 32(1):100-102 SONG X P, CHEN Z W, FANG A N, et al. Experimental study on effect of total flavone of ginkgo biloba on delaying cell senescence[J]. Journal of Chinese Medicinal Materials, 2009, 32(1):100-102
[6] 胡作为, 沈自尹, 黄建华. 淫羊藿总黄酮保护衰老细胞端粒长度缩短的实验研究[J]. 中国中西医结合杂志, 2004, 24(12):1094-1097 HU Z W, SHEN Z Y, HUANG J H. Experimental study on effect of epimedium flavonoids in protecting telomere length of senescence cells[J]. Chinese Journal of Integrated Traditional and Western Medicine, 2004, 24(12):1094-1097
[7] 石丽君, 吴铁, 吴志华. D-半乳糖诱导大鼠皮肤衰老的形态学与生化指标观察[J]. 中华皮肤科杂志, 2005, 38(3):165-167 SHI L J, WU T, WU Z H. Morphology and biochemical parameters in rats with D-galactose induced skin ageing[J]. Chinese Journal of Dermatology, 2005, 38(3):165-167
[8] 曹蕾, 陈逸青, 陈勤, 等. 西施口服液延缓小鼠皮肤衰老的作用[J]. 中国医院药学杂志, 2016, 36(17):1447-1451 CAO L, CHEN Y Q, CHEN Q, et al. Antiaging effects of Xishi oral liquid on D-galactose induced aging mouse model[J]. Chinese Journal of Hospital Pharmacy, 2016, 36(17):1447-1451
[9] 谢晓辉, 朱瑶, 窦智燕, 等. 一种复方口服美容液的抗衰老作用及毒理学实验研究[J]. 中国美容医学, 2017, 26(5):35-38 XIE X H, ZHU Y, DU Z Y, et al. Experimental research on anti-aging effect and acute toxicity of a compound oral liquid[J]. Chinese Journal of Aesthetic Medicine, 2017, 26(5):35-38
[10] 王喜军. 中药血清药物化学的研究动态及发展趋势[J]. 中国中药杂志, 2006, 31(10):789-792, 835 WANG X J. Progress and future developing of the serum pharmacochemistry of traditional Chinese medicine[J]. China Journal of Chinese Materia Medica, 2006, 31(10):789-792, 835
[11] 宋琳琳, 赵宏, 沙靖全, 等. 冬奇子口服液延缓皮肤衰老的实验研究[J]. 时珍国医国药, 2012, 23(6):1344-1346 SONG L L, ZHAO H, SHA J Q, et al. Experimental study on anti-skin-aging action of Dongqi oral liquid[J]. Li Shizhen Medicine and Materia Medica Research, 2012, 23(6):1344-1346
[12] 陈高敏, 王璐, 杜沛, 等. 麦冬多糖对中波紫外线损伤人皮肤成纤维细胞的保护作用[J]. 中国组织工程研究, 2017, 21(32):5183-5188 CHEN G M, WANG L, DU P, et al. Poly saccharide from Ophiopogon japonicus protects against ultraviolet B damage to the human dermal fibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(32):5183-5188
[13] 倪庆纯, 郭健敏, 肖百全, 等. 两种中药组方对D-半乳糖诱导的衰老模型裸鼠皮肤的影响[J]. 中草药, 2012, 43(9):1808-1813 YU Q C, GUO J M, XIAO B Q, et al. Effects of two prescriptions with Chinese herbal medicines on skin of aging nude mouse model reduced by D-galactose[J]. Chinese Traditional and Herbal Drugs, 2012, 43(9):1808-1813
[14] 黄娅琳. 抗衰老中药的研究[J]. 时珍国医国药, 2007, 18(3):691-693 HUANG Y L. Progress in studies of the anti-aging traditional Chinese drugs[J]. Li Shizhen Medicine and Materia Medica Research, 2007, 18(3):691-693
[15] 张玲, 王文君. 中药延缓皮肤老化作用的研究进展[J]. 中西医结合学报, 2009, 7(3):276-279 ZHANG L, WANG W J. Research advances of traditional Chinese medicine in delaying skin aging[J]. Journal of Chinese Integrative Medicine, 2009, 7(3):276-279
[16] 王曦, 石钰, VIENNET C, 等. 黄芪甲苷对人皮肤成纤维细胞增殖和凋亡的影响[J]. 中华医学美学美容杂志, 2006, 12(2):93-97 WANG X, SHI Y, VIENNET C, et al. Effects of astragaloside on growth activity and apoptosis of fibroblasts from human skin with and without wrinkle in vitro[J]. Chinese Journal of Medical Aesthetics and Cosmetology, 2006, 12(2):93-97
[17] 赵蕾, 魏晓东, 葛堂栋, 等. 黄芪血清对衰老细胞抗氧化作用及p16表达影响的研究[J]. 黑龙江医药科学, 2006, 29(5):4-5 ZHAO L, WEI X D, GE T D, et al. Effects of Astragalus Root serum on senile cell anti-oxidation and p16 expression[J]. Heilongjiang Medicine and Pharmacy, 2006, 29(5):4-5
[18] 王奕, 王静凤, 高森, 等. 日本刺参胶原蛋白多肽对紫外线诱导的光老化模型小鼠皮肤的保护作用[J]. 中国药科大学学报, 2008, 39(1):64-67 WANG Y, WANG J F, GAO S, et al. Protective effect of collagen polypeptides from Apostichopus japonicus on the skin of photoaging-model mice induced by ultraviolet irradiation[J]. Journal of China Pharmaceutical University, 2008, 39(1):64-67
[19] ABACI H E, GUO Z, DOUCET Y, et al. Next generation human skin constructs as advanced tools for drug development[J]. Experimental Biology and Medicine, 2017, 242:1657-1668
[20] ALI N, HOSSEINI M, VAINIO S, et al. Skin equivalents:skin from reconstructions as models to study skin development and diseases[J]. British Journal of Dermatology, 2015, 173(2):391-403
No related articles found!
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2017 《广东工业大学学报》编辑部
地址：广州市东风东路729号广东工业大学 邮编：510090
电话：020-37626653,020-37626139,020-37626396
邮箱：xbzrb@gdut.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发