广东工业大学学报 ›› 2013, Vol. 30 ›› Issue (3): 122-126.doi: 10.3969/j.issn.1007-7162.2013.03.024

• 综合研究 • 上一篇    

EDC/NHS交联胶原基牙周组织引导再生膜材料的研究

季培红1,2，侯思润1,2，林绣丹1,2，任力2,3，刘卅2,3*   

  1. 1．广州华美康联生物科技有限公司，广东 广州 510000；华南理工大学 2．材料科学与工程学院，3．国家人体组织功能重建工程技术研究中心，广东 广州 510641
  • 收稿日期:2013-03-20 出版日期:2013-09-30 发布日期:2013-09-30
  • 作者简介:季培红(1984)，男，工程师，硕士，主要研究方向为医用材料的制备和改性.
  • 基金资助:

    国家863计划项目(2011AA030105);“十二五”国家科技支撑计划项目（2012BAI17B02）;国家自然科学基金资助项目（51273072；50803018），广东省战略新兴产业核心技术攻关项目（2012A080800015）；广东省重大科技专项资助项目（2010A080407008）

Cross linked Collagen Guided Tissue Regeneration Membrane with EDC/NHS

Ji Pei-hong1,2, Hou Si-run1,2, Lin Xiu-dan1,2, Ren Li2,3, Liu Sa2,3*   

  1. 1. HM Biotechnology，Co., Ltd.，Guangzhou 510000， China; 2. College of Materials Science and Engineering, 3. National Engineering Research center for Tissue Regeneration and Reconstruction, South China University of Technology，Guangzhou 510641，China
  • Received:2013-03-20 Online:2013-09-30 Published:2013-09-30

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摘要： 采用胶原材料制备了一种密实-疏松双层结构的牙周引导再生膜材料.为改善再生膜材料的物理化学性能，采用1-乙基-3-(3-二甲基氨丙基)碳二亚胺（EDC）和N-羟基琥珀酰亚胺(NHS)为交联剂对膜材料进行交联，考察了不同交联剂质量浓度对膜材料物理化学性能的影响，并通过差示扫描量热分析仪(DSC)、扫描电镜(SEM)、吸水率测试、膨胀动力学分析、抗酶解性能分析等手段对膜材料交联前后的结构与性能进行了研究.结果表明， 以EDC/NHS为交联剂，在pH为5.5、EDC质量浓度为5 g/L、交联时间为24 h的条件下，引导再生胶原膜材料的综合性能达到最佳，进一步提高交联剂的浓度，材料物理化学性能的变化并不明显.采用EDC/NHS交联后，可显著改善再生膜材料的物理化学性能.交联后膜材料的变性温度和抗酶解性能显著提高，并且维持了密实-疏松的双层结构.

关键词: 组织引导再生膜；胶原；交联；EDC/NHS

Abstract: A collagen GTR membrane was made with a bi-layer structure, one of whose face is loose while the other is tight. The tight face can effectively prevent the cells from growing into the defective tissue zone while the loose face is rough, which is helpful to cells growth and blood clot. In order to improve the physicochemical properties of the membranes, EDC/NHS was used as a cross-linking agent for the membranes. And the effects of cross-linking agent dosage on the physicochemical properties of membranes were investigated. The measurements of DSC, SEM were used to test the structure and properties of the membranes. The properties of water absorption, collagen resistance were also tested. According to the study, 0.5 mol/L EDC/NHS, cross-linking 24 h in the pH of 5.5 are the best processing conditions. Increasing further the concentration of EDC can not improve the physicochemical properties of the membranes. The research shows that the physicochemical properties of collagen membranes are improved significantly after being crosslinked. The denaturation temperature, anti-collagen activities are both increased, and the bi-layer structure is kept well.

Key words: GTR membrane; collagen; crosslinking; EDC/NHS

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