Abstract: In order to better understand the cycling jersey color collocation preference with different customers and provide relevant clothing enterprises with some valuable reference on designing and developing, the fixation duration and beginning time of the various areas of different samples are recorded by eye-tracking apparatus and analyzed with SPSS (Statistical Product and Service Solutions) by the means of variance based on analysis of cycling brand and questionnaire survey, taking cycling jerseys with two and three kinds of color collocation as experimental samples. In two kinds of color collocation, with the same background color and different ornament color, the result shows that there is no significant difference in fixation duration between different customers. However, with different background color and the same ornament color, the fixation duration would be quite different. Thus, in two kinds of color collocation, customers would pay more attention to the choice of background colors. Also, the result shows that, in three kinds of color collocation, where the background color is the same and the ornament color is different, the intensive correlation exists between fixation duration and customers, which shows that customers have different ornament color preferences for different background colors.