广东工业大学学报 ›› 2013, Vol. 30 ›› Issue (1): 115-119.doi: 10.3969/j.issn.1007-7162.2013.01.022

• 综合研究 • 上一篇    

响应面法优化马尾松松针中总黄酮提取工艺

张卫丽，韩雅莉   

  1. 广东工业大学 轻工化工学院，广东 广州 510006
  • 收稿日期:2012-04-19 出版日期:2013-03-30 发布日期:2013-03-30
  • 作者简介:张卫丽（1988-），女，硕士研究生，主要研究方向为天然药物及其药理学.
  • 基金资助:

    国家自然科学基金资助项目（30772739）

The Extraction Process of  Pinus Massoniana Optimized by Response Surface

Zhang Wei-li, Han Ya-li   

  1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China
  • Received:2012-04-19 Online:2013-03-30 Published:2013-03-30

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摘要： 采用超声波辅助的方法从马尾松松针中提取总黄酮，比较了乙醇体积分数、提取时间、料液比等因素对提取率的影响，采用响应面分析法对松针提取物工艺条件进行优化.实验结果表明，松针总黄酮提取的最佳工艺条件为：提取时间56 min、料液比1:20、乙醇体积分数40%、功率90%、提取率1.59%.在响应面法工艺优化实验所选取的探索因素中，乙醇体积分数、功率两因素对此马尾松松针总黄酮的提取影响较为显著.

关键词: 马尾松；超声波提取；黄酮；响应面优化实验设计

Abstract: Ultrasonic was employed to extract flavonoids from Pinus massoniana. Conditions for extraction were influenced by some factors, such as ethanol concentration,time and liquid-material ration, so they were optimized by response surface. Experimental results show the best conditions are as follows: the extracting time is 56 minutes,ethanol concentration is 40%, liquidmaterial ration equals 1:20, when the yield of flavonoids is 1.59%.This method is feasible for exploiting the material, and it provides the basis for its utilization. In the Response Surface experiment, compared with the factors of liquid-material ration and extracting time,ethanol concentration and power have greater influence on the extraction.

Key words: Pinus massoniana; ultrasonic extraction; flavonoids; response surface optimization

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