响应面优化弹性蛋白酶酶解罗非鱼下脚料的工艺研究

doi:10.3969/j.issn.1007-7162.2016.05.014

广东工业大学学报 ›› 2016, Vol. 33 ›› Issue (05): 77-82.doi: 10.3969/j.issn.1007-7162.2016.05.014

响应面优化弹性蛋白酶酶解罗非鱼下脚料的工艺研究

瞿叶辉¹，丁利君¹，彭文媛¹，刘珊珊¹，黄金²，成晓玲¹

广东工业大学 1.轻工化工学院；2.材料与能源学院，广东广州 510006

收稿日期:2015-10-20 出版日期:2016-09-10 发布日期:2016-09-10
通信作者: 丁利君（1965-），女，教授，主要研究方向为农产品加工与天然产物化学.E-mail: ddddlj@foxmail.com
作者简介:瞿叶辉（1990-），女，硕士研究生，主要研究方向为食品加工.
基金资助:
广东省教育部产学研合作重大专项(2013A090100009).

Optimization of Enzymolysis of Tilapia Waste with Response Surface Methodology by Elastase

Qu Ye-hui¹, Ding Li-jun¹, Peng Wen-yuan ¹, Liu Shan-shan¹, Huang Jin ², Cheng Xiao-ling¹

1.School of Chemical Engineering and Light Industry; 2. School of Materials and Energy, Guangdong University of Technology, Guangzhou 510006, China

Received:2015-10-20 Online:2016-09-10 Published:2016-09-10

摘要/Abstract

摘要：

以罗非鱼加工鱼片的下脚料鱼头、鱼骨、鱼尾等为原料，选用弹性蛋白酶对其水解，通过响应面进行分析并优化酶解条件.以水解度与多肽含量为指标，分别对酶浓度、温度、pH值、料液比、酶解时间等因素对弹性蛋白酶酶解罗非鱼下脚料水解效果的影响进行分析.结果表明,弹性蛋白酶水解罗非鱼加工下脚料的最佳条件为：酶浓度0.553 mg/g，料液比1:5、自然pH、温度41.94 ℃、酶解3.31 h，在此条件下得到的水解度为18.09%，多肽含量为0.9 mg/mL.

关键词: 罗非鱼下脚料；响应面分析；弹性蛋白酶；水解度；多肽含量

Abstract:

The head, bones, and other raw waste of Tilapia are hydrolyzed by elastase, and response surface analysis method is used to optimize the enzymatic processes. The enzyme concentration, temperature, pH, solid to liquid ratio, time and other factors are discussed to analyze the effect of hydrolysis based on the degree of hydrolysis and peptide content. The results show that the optimum conditions of elastase hydrolysis: enzyme concentration 0.553 mg/g, temperature 41.94 ℃, time 3.31 h, pH natural, solid-liquid ratio 1: 5, the degree of hydrolysis and the peptide content is separately 18.09% and 0.90 mg /ml in this condition.

Key words: Tilapia waste; response surface methodology; elastase；hydrolysis; peptide

瞿叶辉, 丁利君, 彭文媛, 刘珊珊, 黄金, 成晓玲. 响应面优化弹性蛋白酶酶解罗非鱼下脚料的工艺研究[J]. 广东工业大学学报, 2016, 33(05): 77-82.

QU Ye-Hui, DING Li-Jun, PENG Wen-Yuan, LIU Shan-Shan, HUANG Jin, CHENG Xiao-Ling. Optimization of Enzymolysis of Tilapia Waste with Response Surface Methodology by Elastase[J]. Journal of Guangdong University of Technology, 2016, 33(05): 77-82.

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响应面优化弹性蛋白酶酶解罗非鱼下脚料的工艺研究

Optimization of Enzymolysis of Tilapia Waste with Response Surface Methodology by Elastase

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