黄粉虫纤溶活性蛋白免疫原性与抗血栓作用的研究

doi:10.3969/j.issn.1007

广东工业大学学报 ›› 2015, Vol. 32 ›› Issue (2): 144-148.doi: 10.3969/j.issn.1007

• 综合研究 • 上一篇

黄粉虫纤溶活性蛋白免疫原性与抗血栓作用的研究

张冰, 谭竹钧

广东工业大学轻工化工学院，广东广州 510006

收稿日期:2014-01-02 出版日期:2015-05-30 发布日期:2015-05-30
作者简介:张冰（1988-），男，硕士研究生，主要研究方向为生化药物.
基金资助:
国家自然科学基金资助项目（30772739）；2011年海口市重点科技计划项目(20110044)

The Study of Immuongenicity and Antithrombotic Effect of Tenebrio Fibrinolyric Proteins

Zhang Bing，Tan Zhu-jun

School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China

Received:2014-01-02 Online:2015-05-30 Published:2015-05-30

摘要/Abstract

摘要： 为了研究黄粉虫纤溶活性蛋白(Tenebrio Fibrinolytic Proteins,TFP)的免疫原性及其抗血栓作用，本研究将纯化的TFP经皮下注射免疫小鼠，间接ELISA法检测小鼠血清中的抗体含量及抗体效价；小鼠灌胃TFP后，取血浆检测凝血酶时间(thrombin time,TT)、凝血酶原时间(prothrombin time,PT)、活化部分凝血活酶时间(activated partial thromboplastin time,APTT)、组织型纤溶酶原激活剂(tissuetype plasminogen activator,tPA)和其抑制物(plasminogen activator inhibitor,PAI)，角叉菜胶使小鼠尾部形成血栓，测量血栓形成长度.结果显示，TFP免疫小鼠后产生特异性抗体且随着免疫次数的增加，抗体含量逐渐升高，末次免疫后产生的抗体效价为1∶ 640；TFP可以显著延长TT，增强t-PA的活性，抑制PAI的活性，同时显著抑制由角叉菜胶引起的小鼠尾部血栓的形成.这说明TFP具有良好的免疫原性且具有一定的抗血栓作用.

关键词: 黄粉虫；纤溶活性蛋白；免疫原性；抗血栓

Abstract: To study the immuongenicity and antithrombotic effect of Tenebrio Fibrinolyric Proteins(TFP), mice were immunized with purified TFP by subcutaneous injection, then the antibody content and antibody titer were detected by the optimal ELISA method. Mice were gavaged with purified TFP, then thrombin time(TT), prothrombin time(PT), activated partial thromboplastin time(APTT), tissuetype plasminogen activator(tPA) and plasminogen activator inhibitor(PAI) activity were detected.The results show that the specific antibody is found after TFP immunized mice and the antibody content increases when the number of immunization rises. The specific antibody titer is 1∶640 after the last immunization.The TT of mice is remarkably prolonged after using TFP. The tPA activity increases while the PAI activity decreases. Meanwhile the average thrombosis length of tail is significantly reduced.This shows that the TFP has good immunogenicity and antithrombotic effect.

Key words: tenebrio molitor；fibrinolytic proteins； immunogenicity； antithrombotic

张冰, 谭竹钧. 黄粉虫纤溶活性蛋白免疫原性与抗血栓作用的研究[J]. 广东工业大学学报, 2015, 32(2): 144-148.

ZHANG Bing, TAN Zhu-Jun. The Study of Immuongenicity and Antithrombotic Effect of Tenebrio Fibrinolyric Proteins[J]. Journal of Guangdong University of Technology, 2015, 32(2): 144-148.

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黄粉虫纤溶活性蛋白免疫原性与抗血栓作用的研究

The Study of Immuongenicity and Antithrombotic Effect of Tenebrio Fibrinolyric Proteins

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