广东工业大学学报 ›› 2021, Vol. 38 ›› Issue (02): 88-93.doi: 10.12052/gdutxb.200028
刘迪1, 郭清泉1, 赵海山2, 舒均中3, 练英铎3, 夏树敏3
Liu Di1, Guo Qing-quan1, Zhao Hai-Shan2, Shu Jun-zhong3, Lian Ying-duo3, Xia Shu-min3
摘要: 用体外药敏试验评价了丁香酚抗马拉色菌的活性, 并通过棋盘微量稀释法研究了丁香酚与传统去屑剂联合用药的相互作用, 丁香酚的最低抑菌浓度(Minimum Inhibitory Concentration, MIC)为312.50 μg/mL, 最低杀菌浓度(Minimum Fungicidal Concentration, MFC)为625.00 μg/mL; 与酮康唑联用表现为协同作用, 与吡啶硫酮锌 (Zinc Pyrithione, ZPT)、氯咪巴唑、吡啶酮乙醇胺盐 (Piroctone Olamine, OCT)分别联用表现为相加作用;用四甲基偶唑盐 (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromid,MTT)法评价了丁香酚对角质形成细胞的毒性, 其半抑制浓度(50% Inhibitory Concentration,IC50)为320.93 μg/mL, 高于传统去屑剂;最后通过扫描电子显微镜 (Scanning Electron Microscope, SEM) 和透射电子显微镜 (Transmission Electron Microscope, TEM) 初步研究了丁香酚对马拉色菌的作用机制, 结果表明丁香酚通过破坏细胞壁和细胞内部膜结构达到杀菌的效果。
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[1] XU J, SAUNDERS C W, HU P, et al. Dandruff-associated Malassezia genomes reveal convergent and divergent virulence traits shared with plant and human fungal pathogens [J]. Proceedings of the National Academy of Sciences, 2007, 104(47): 18730-18735. [2] HARADA K, SAITO M, SUGITA T, et al. Malassezia species and their associated skin diseases [J]. The Journal of Dermatology, 2015, 42(3): 250-257. [3] WHITE T C, FINDLEY K, DAWSON T L, et al. Fungi on the skin: dermatophytes and Malassezia [J]. Cold Spring Harbor Perspectives in Medicine, 2014, 4(8): a019802. [4] GRICE E A, DAWSON T L. Host-microbe interactions: Malassezia and human skin [J]. Current Opinion in Microbiology, 2017, 40: 81-87. [5] CLAVAUD C, JOURDAIN R, BAR-HEN A, et al. Dandruff is associated with disequilibrium in the proportion of the major bacterial and fungal populations colonizing the scalp [J]. PloS One, 2013, 8(3): e58203. [6] SCHWARTZ J R, VALENZUELA M, MIDHA S. Shampoos for normal scalp hygiene and dandruff [M]. Hoboken: Wiley-Black Well, 2010. [7] ANGIOLELLA L, CARRADORI S, MACCALLINI C, et al. Targeting Malassezia species for novel synthetic and natural antidandruff agents [J]. Current Medicinal Chemistry, 2017, 24(22): 2392-2412. [8] CHEN Z F, YING G G. Occurrence, fate and ecological risk of five typical azole fungicides as therapeutic and personal care products in the environment: a review [J]. Environment International, 2015, 84: 142-153. [9] 成秋桂, 罗佩玲, 卢珊. 去屑产品的新方向[J]. 日用化学品科学, 2017, 40(3): 23-25. CHENG Q G, LUO P L, LU S. New directions of anti-dandruff products [J]. Detergent & Cosmetics, 2017, 40(3): 23-25. [10] CHANDRANI D, LUBAINA S Z, SOOSAMMA M. A review of antifungal effect of plant extract vs chemical substances against Malassezia spp [J]. International Journal of Pharma and Bio Sciences, 2012, 3(3): 773-780. [11] KAMATOU G P, VERMAAK I, VILJOEN A M. Eugenol—from the remote Maluku Islands to the international market place: a review of a remarkable and versatile molecule [J]. Molecules, 2012, 17(6): 6953-6981. [12] VÁCZI P, ČONKOVÁ E, MARCINČÁKOVÁ D, et al. Antifungal effect of selected essential oils on Malassezia pachydermatis growth [J]. Folia Veterinaria, 2018, 62(2): 67-72. [13] SHAHI S K, SHAHI M P, PRAKASH D. Syzygium aromaticum: potent antidandruff agent with thermo-tolerance, quick killing action and long shelf life [J]. Indo American Journal of Pharmaceutical Research, 2015, 5(2): 795-802. [14] ESSID R, HAMMAMI M, GHARBI D, et al. Antifungal mechanism of the combination of Cinnamomum verum and Pelargonium graveolens essential oils with fluconazole against pathogenic Candida strains [J]. Applied Microbiology and Biotechnology, 2017, 101(18): 6993-7006. [15] CAI R, HU M, ZHANG Y, et al. Antifungal activity and mechanism of citral, limonene and eugenol against Zygosaccharomyces rouxii [J]. LWT-Food Science and Technology, 2019, 106: 50-56. [16] DE OLIVEIRA PEREIRA F, MENDES J M, DE OLIVEIRA LIMA E. Investigation on mechanism of antifungal activity of eugenol against Trichophyton rubrum [J]. Medical Mycology, 2013, 51(5): 507-513. [17] DARVISHI E, OMIDI M, BUSHEHRI A A S, et al. The antifungal eugenol perturbs dual aromatic and branched-chain amino acid permeases in the cytoplasmic membrane of yeast [J]. PloS One, 2013, 8(10): e76028. |
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