广东工业大学学报 ›› 2012, Vol. 29 ›› Issue (3): 87-91.doi: 10.3969/j.issn.1007-7162.2012.03.017

• 综合研究 • 上一篇    下一篇

一种改良的纤维平板法

黄明星，叶韵，谭竹钧，韩雅莉   

  1. 广东工业大学 轻工化工学院，广东 广州 510006
  • 收稿日期:2012-02-16 出版日期:2012-09-20 发布日期:2012-09-20
  • 作者简介:黄明星（1982-），男，中级实验员，博士，主要研究方向为蛋白质的分离技术、溶栓活性蛋白及溶栓药物的开发.
  • 基金资助:

    海口市重点科技计划项目（2011-0044）

An Improved Fiber Plate Method

Huang Mingxing, Ye Yun, Tan Zhujun, Han Yali   

  1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China
  • Received:2012-02-16 Online:2012-09-20 Published:2012-09-20

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摘要： 介绍一种改良的用以快速地定性和半定量检测纤溶活性蛋白的纤维平板法.对该方法中的几个关键因素作了详细的研究和探讨.纤维平板中纤维蛋白原和纤溶酶原的浓度对裂解面积不产生任何影响，但是纤维蛋白原的浓度会对裂解圈的观察产生影响，纤维蛋白原浓度低于02 mg/mL时会使背景色过淡而不能清晰地观察到裂解圈.尿激酶浓度在2~250 mU/μL范围时，尿激酶浓度的对数和裂解圈相互垂直的两条直径乘积的对数之间存在明显的线性关系.在本实验设计方案下，尿激酶的最佳使用浓度为10~20 mU/μL.同时，本文还探讨了纤维平板中纤溶酶原的去活条件，当琼脂纤维平板凝固后很难完全去活纤溶酶原，但是，在琼脂纤维蛋白原混合物凝固前于95 ℃持续温浴40 min，则能彻底去活纤维蛋白原中残留的纤溶酶原.

关键词: 纤维平板法;纤维蛋白原;纤溶酶原;尿激酶

Abstract: It establishes an improved fiber plate method, which can be used for qualitative and semiquantitative analysis. Several key factors were studied and discussed. The concentration of fibrinogen and plasminogen contained in the fibrin plate did not have any effect on the fibrinolytic area. However, the concentration of fibrinogen had influence on the observation and image gathering of fibrinolytic zones. It was difficult to clearly observe the fibrinolytic zones when the concentration of fibrinogen was below 02 mg/mL. The logarithm of the product of the two perpendicular diameters was linearly related to the logarithm of the concentration of urokinase when the concentration of urokinase was among 2~250 mU/μL. In the experiment, the optical concentration of urokinase was among 10~20 mU/μL. It is found that it is hard to denature plasmin by heating the agarfibrin plate after the agar is solidified. However, the plasmin is completely denatured when heated at 95 ℃ for 40 minutes before the agar is solidified.

Key words: fibrin plate method; fibrinogen; plasminogen; urokinase

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