Journal of Guangdong University of Technology ›› 2023, Vol. 40 ›› Issue (01): 130-136.doi: 10.12052/gdutxb.210155

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Protective Effect of Schisandra Chinensis Oil on H2O2-induced Oxidative Stress Injury in HaCaT Cells by 3D Skin Model

Tai Mei-ling1, Jiang Ling2, Li Yuan-zhao1, Han Ping3, Lin Li3, Du Zhi-yun2   

  1. 1. Infinitus (China) Co., Ltd., Jiangmen 529000, China;
    2. School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China;
    3. Foshan Allan Conney Biotechnology Co., Ltd., Foshan 528231, China
  • Received:2021-10-21 Online:2023-01-25 Published:2023-01-12

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Abstract: The protective effect of Schisandra chinensis oil on H2O2-induced oxidative damage of HaCaT cells is evaluated by 3D skin model. Schisandra chinensis oil was extracted by steam distillation, and its chemical constituents analyzed by GC-MS. And the antioxidant activity of schisandra chinensis oil was evaluated by DPPH and ABTS free radical scavenging experiments, combined with 2D and 3D skin models induced by H2O2. The results showed that Schisandra chinensis oil mainly contained 37 compounds including ylenene, β-cimarene and L-α-pinene. Schisandra chinensis oil had good scavenging effect on DPPH and ABTS free radicals, with IC50 of 5.6 mg/mL and 9.4 mg/mL, respectively. What’s more, Schisandra chinensis oil can improve the survival rate of HaCaT cells after H2O2 treatment, increasing the epidermal thickness of 3D skin model, decreasing the level of Interleukin-6(IL-6), and up-regulating the activities of SOD and CAT enzymes. The results confirmed that Schisandra chinensis oil had protective effect on H2O2-induced oxidative stress injury model of HaCaT cells, which could lay a foundation for the development of anti-aging products of schisandra chinensis oil.

Key words: Schisandra chinensis oil, hydrogen peroxide, antioxidant, HaCaT cell, 3D skin model

CLC Number: 

  • R965.1
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