Abstract:

In order to explore the role of Notch1 signaling pathway in ERα-induced proliferation of NCI-H23, this research treats non-small cell lung cancer cells with E2, DATP or siRNA and analyzes the proliferation and protein expression of NCI-H23 by MTT, colony assay, PI assay and western blotting. The results show that E2 can enhance the proliferative ability with the proportion of NCI-H23 at S phase 1.33 fold of the controlled group. The expressions of Bcl-2 and PCNA in E2-treated cells are higher than the non-treated and Bax expression is decreased. The proliferative ability of DATP combined with E2 treatment declines evidently with P＜0.01 and the cell proliferative proportion 0.66 time of E2 group. With the obvious increase of BAX expression and the decrease of cell viability, before treatment with E2 transfection Notch1 siRNA in NCI-H23, the results show that ERα expression is lower than the controlled siRNA. Thus it draws the conclusion that Notch signaling affects the E2-stimulated cell proliferation and the inhibition of Notch signaling can impair the influence of E2 in NCI-H23.

Key words: Notch1 signaling pathway; estrogen receptor; non-small cell lung cancer