Journal of Guangdong University of Technology ›› 2006, Vol. 23 ›› Issue (3): 21-25.

• Comprehensive Studies • Previous Articles     Next Articles

Construction of Thin-layer Chromatography Scan (TLCS) Fingerprinting for Ethyl Acetate Extract of Compound Decoction of Yinchenhao and Huanglianjiedu(CDYH)

  

  1. (1.Facultg of Chemistry Engineering and Light Industry,Guangdong University of Technology,Guangzhou 510090,China;2.Nanfang Hospital,Southern Medical University,Guangzhou 510515,China)
  • Online:2006-06-02 Published:2006-06-02

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Abstract: A TLCS fingerprinting is established for ethyl acetate extract of CDYH,which will do a great deal of good for the quality standard of CDYH.The ethyl acetate extract is chromatographed by TLC with benzene∶ethyl acetate∶methanol∶formic acid（5∶1∶1.5∶5drops） and its chromatography is scanned by CS9000 TLCS at λs= 275nm and λR=365nm or λ= 435nm.A good separating TLCS fingerprinting is established,four peaks of which are identified as berberine,caffeic acid,ferulic acid and rhein.The method is simple and rapid with good precision and reproducibility for quality control of this preparation.

Key words: compound decoction of yinchenhao and huanglianjiedu(CDYH)； TLCS； fingerprinting；

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