Journal of Guangdong University of Technology ›› 2017, Vol. 34 ›› Issue (02): 23-27.doi: 10.12052/gdutxb.160090

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An Optimized Design for Protease Produced by Aspergillus Niger Liquid-state Fermentation

Li Xing-hua, Huang Zi-hao, Zhang Chi, Bi Yan-mei, Ding Li-jun, Huang De-mei, Liu Dan   

  1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China
  • Received:2016-06-30 Online:2017-03-09 Published:2017-03-09

Abstract:

Different proportion and combination of bran, corn flour and soybean powder was applied to optimize the best composition of culture medium. Single factor experiments and four factors and three levels orthogonal test were used to improve the fermentation conditions. The result showed that the optimal composition of culture medium was 4 g bran, 1 g soybean powder, 4 g ammonium sulphate, 0.3 g dipotassium hydrogen phosphate and 0.5 g calcium chloride, the protease production was maximized under the conditions with an addition of water at 45℃ for 120 h with pH 7. The optimal fermentation conditions obtained were the initial pH of 7.0, 125 mL addition of water, cultivation temperature of 45℃, and cultivation time of 120 h. Under this condition, the best protease activity of 269.68 U/mL was obtained.

Key words: Aspergillus niger, protease, liquid state fermentation

CLC Number: 

  • TS201.3

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