石斛多糖提取和对体外黑色素抑制、抗干燥损伤的影响

doi:10.12052/gdutxb.210194

Abstract

Abstract: In this study, the polysaccharide was extracted by hot water extraction from Dendrobium officinale. The polysaccharide extraction rate was used as an indicator. The ratio of extraction material to liquid, the extraction temperature, extraction times and the extraction time were the influencing factors. A single factor experiment was designed. A better extraction process was obtained through orthogonal experiments. The extraction rate of polysaccharides was 12.45%, and the optimal extraction conditions were: extraction time is 60 min, material-liquid ratio is 1: 20, extraction times are 2 times, and extraction temperature is 60 ℃. The effects of polysaccharides on cell viability, tyrosinase activity and melanin content were determined by B16 cells experiment. By establishing a dry injury model in HaCaT cells, HaCaT cells dry injury model was established, and the cell viability of polysaccharides on cells in dry injury was determined. Different concentrations of polysaccharides (10, 62.5, 125, 250, 500 μg/mL) can inhibit melanin production, in the polysaccharide concentration of 500 μg/mL, the tyrosinase activity was 69.30% and the melanin content was 66.20%. Different concentrations of polysaccharides (10, 62.5, 125, 250, 500 μg/mL) can reduce cell desiccation damage, and in the polysaccharide concentration of 125 μg/mL, the survival rate of cell desiccation damage increases by 15.51% to 86.58%. Dendrobium officinale polysaccharide can reduce the melanin content by inhibiting tyrosinase activity, and can increase the survival rate of cells in desiccation damage and reduce the death of cells caused by desiccation damage. With the establishment of the HaCaT cells desiccation damage model, polysaccharides can improve the survival rate of cells in desiccation damage and reduce the death of cells caused by desiccation damage.

Key words: Dendrobium polysaccharides, extraction process, anti-drying damage, melanogenesis, tyrosinase

CLC Number:

TQ658

Wei Meng-yao, Zhang Xiao-hui, Li Fei, Wu Zi-feng, Yang Yong-zhi, Ding Jin-long. Extraction of Dendrobium Polysaccharides and Its Effects on Inhibition of Melanin and Resistance to Drying Damage in Vitro[J].Journal of Guangdong University of Technology, 2023, 40(03): 91-98.

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