Journal of Guangdong University of Technology ›› 2012, Vol. 29 ›› Issue (3): 77-80.doi: 10.3969/j.issn.1007-7162.2012.03.015

• Comprehensive Studies • Previous Articles     Next Articles

A Study of Bacterium Growth Curve and Suspended Matter in the Medium

Du Qingping,  Wang Qian,  Cao Lichuang,  Zhang Xiaotong, Zeng Qihao,  Deng Tongchu   

  1. School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China
  • Received:2011-05-20 Online:2012-09-20 Published:2012-09-20

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Abstract: The bacterium growth curve was determined via the two methods, surviving bacteria count and spectrophotometry, used to study the growth characteristics of a bacterium called JS17. The results obtained by means of the surviving bacteria count can reflect the actual number of live bacteria，but this method needs many operations and it is timeconsuming. It is more convenient to use the spectrophotometry method, but the results got by means of this method were influenced by other suspended matter, which were possibly the chemical reaction materials or metabolism products of JS17. The growth curve of JS17, with shaking culture in 2# oil medium, shows that lag phase in 0~20 h，log phase in 20~48 h, stationary phase in 48~72 h and decline phase emerge after 72 h. The results suggest that the suspended matter affects the rule of growth curve. The metabolism of the suspended matter of bacterium JS17 deserves further study.

Key words: growth curve; surviving bacteria count; spectrophotometry mehod

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