Journal of Guangdong University of Technology ›› 2012, Vol. 29 ›› Issue (3): 77-80.doi: 10.3969/j.issn.1007-7162.2012.03.015

• Comprehensive Studies • Previous Articles     Next Articles

A Study of Bacterium Growth Curve and Suspended Matter in the Medium

Du Qingping,  Wang Qian,  Cao Lichuang,  Zhang Xiaotong, Zeng Qihao,  Deng Tongchu   

  1. School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China
  • Received:2011-05-20 Online:2012-09-20 Published:2012-09-20

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Abstract: The bacterium growth curve was determined via the two methods, surviving bacteria count and spectrophotometry, used to study the growth characteristics of a bacterium called JS17. The results obtained by means of the surviving bacteria count can reflect the actual number of live bacteria，but this method needs many operations and it is timeconsuming. It is more convenient to use the spectrophotometry method, but the results got by means of this method were influenced by other suspended matter, which were possibly the chemical reaction materials or metabolism products of JS17. The growth curve of JS17, with shaking culture in 2# oil medium, shows that lag phase in 0~20 h，log phase in 20~48 h, stationary phase in 48~72 h and decline phase emerge after 72 h. The results suggest that the suspended matter affects the rule of growth curve. The metabolism of the suspended matter of bacterium JS17 deserves further study.

Key words: growth curve; surviving bacteria count; spectrophotometry mehod

［1］ 张艳，戴晶平．凡口铅锌矿选矿废水资源化研究与应用［J］．有色金属:选矿部分，2007(6): 3335．

［2］ 栾和林，姚文．矿山化学药剂污染及其复合污染问题与探讨［J］．矿冶，2002，11（7）：265267．

［3］ Gao Ying, Chu Weiwei, Zhang Xia, et al. Isolation and determination of growth curve of deoderizing microorganism from swine manure［J］. Journal of Mountain Agriculture and Biology，2011，30 (1)： 12-14．

［4］ 代群威，李琼芳．环境工程微生物学实验［M］．北京：化学工业出版社，2009:1215．

［5］ 李庆忠，张忠智，王洪君，等．一株代谢煤油兼性厌氧菌的筛选鉴定和特性［J］．微生物学通报，2002，29（3）：134-135．

［6］ 李志恒，王廷英，朱正祥,等．解吸水解废液中微量尿素分析方法讨论［J］．化肥设计，2011，49(2)：31-33．

［7］ 熊海燕，李莹．不同果汁发酵液中酵母菌生长曲线的测定及pH值的变化［J］．农产品加工学刊，2009(4)：26-27．

［8］ 李华，张世华，班春兰． 烟气脱硫菌株生长曲线的测定及生理生化试验［J］． 洁净煤技术，2007,13(1)：44-45．

［9］ 辛华，马武．现代细胞生物学技术［M］．北京：科学出版社，2009: 142-143

［10］ 周德庆．微生物学教程［M］．北京：高等教育出版社，2010: 156-157．

［11］  刘婕，杨博．一株高效油脂降解菌的分离鉴定及其性能研究［J］．中国油脂，2010，35(1)：41-43．

［12］ Zhang Guoliang, Wu Yueting, Qian Xinping, et al. Biodegradation of crude oil by pseudomonas aeruginosa in the presence of rhamnolipids［J］．Journal of Zhejiang University，2005，6(8)：725-730．

［13］ R E.布坎南，N E.吉本斯.伯杰细菌鉴定手册［M］.8版．北京：科学出版社，1984．

［14］ 熊宇迪，刘霞，冯长根．杂多酸(蓝)分光光度法在测定痕量元素中的研究进展［J］．光谱学与光谱分析，2008，28(1)：231232．

［15］ Anthony Arnoldo, Jasna Curak,  Kittanakom Saranya，et al. Identification of small molecule inhibitors of pseudomonas aeruginosa exoenzyme S using a yeast phenotypic screen［J］．PLoS Genet,2008，4(2)：346327．
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