Journal of Guangdong University of Technology ›› 2012, Vol. 29 ›› Issue (3): 87-91.doi: 10.3969/j.issn.1007-7162.2012.03.017

• Comprehensive Studies • Previous Articles     Next Articles

An Improved Fiber Plate Method

Huang Mingxing, Ye Yun, Tan Zhujun, Han Yali   

  1. School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China
  • Received:2012-02-16 Online:2012-09-20 Published:2012-09-20

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Abstract: It establishes an improved fiber plate method, which can be used for qualitative and semiquantitative analysis. Several key factors were studied and discussed. The concentration of fibrinogen and plasminogen contained in the fibrin plate did not have any effect on the fibrinolytic area. However, the concentration of fibrinogen had influence on the observation and image gathering of fibrinolytic zones. It was difficult to clearly observe the fibrinolytic zones when the concentration of fibrinogen was below 02 mg/mL. The logarithm of the product of the two perpendicular diameters was linearly related to the logarithm of the concentration of urokinase when the concentration of urokinase was among 2~250 mU/μL. In the experiment, the optical concentration of urokinase was among 10~20 mU/μL. It is found that it is hard to denature plasmin by heating the agarfibrin plate after the agar is solidified. However, the plasmin is completely denatured when heated at 95 ℃ for 40 minutes before the agar is solidified.

Key words: fibrin plate method; fibrinogen; plasminogen; urokinase

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