Abstract: To study the immuongenicity and antithrombotic effect of Tenebrio Fibrinolyric Proteins(TFP), mice were immunized with purified TFP by subcutaneous injection, then the antibody content and antibody titer were detected by the optimal ELISA method. Mice were gavaged with purified TFP, then thrombin time(TT), prothrombin time(PT), activated partial thromboplastin time(APTT), tissuetype plasminogen activator(tPA) and plasminogen activator inhibitor(PAI) activity were detected.The results show that the specific antibody is found after TFP immunized mice and the antibody content increases when the number of immunization rises. The specific antibody titer is 1∶640 after the last immunization.The TT of mice is remarkably prolonged after using TFP. The tPA activity increases while the PAI activity decreases. Meanwhile the average thrombosis length of tail is significantly reduced.This shows that the TFP has good immunogenicity and antithrombotic effect.

Key words: tenebrio molitor；fibrinolytic proteins； immunogenicity； antithrombotic